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Chemical Probe Design & Bioconjugate Synthesis

Introduction

Bio-orthogonal chemistry enables the reaction of two reagents to occur in the presence of a myriad of other reactive groups present in the cellular environment. We are developing new bio-orthogonal reactions that are fast, produce a single product (i.e., no regioisomers) and can be used in the presence of other bio-orthogonal reagents. These reactions are being used to prepare protein and nucleic acid-based bioconjugates and as chemical probes in cell biology.

Related Publications

111. Biocatalytic Synthesis of Ribonucleoside Analogues Using Nucleoside Transglycosylase-2
December 11, 2024
109. Assessing the Manufacturability and Critical Quality Attribute Profiles of Anti-IL-8 Immunoglobulin G Mutant Variants
November 7, 2024
107. Reactivity Profiling for High-Yielding Ynamine-Tagged Oligonucleotide Click Chemistry Bioconjugations
October 10, 2024
105. A First Insight into the Developability of an Immunoglobulin G3: A Combined Computational and Experimental Approach
July 15, 2024
104. A framework for the biophysical screening of antibody mutations targeting solvent-accessible hydrophobic and electrostatic patches for enhanced viscosity profiles
May 23, 2024
103. Mechanistic Basis of the Cu(OAc)2 Catalyzed Azide-Ynamine (3 + 2) Cycloaddition Reaction
May 7, 2024
102. Innovative, combinatorial and high-throughput approaches to degrader synthesis
April 10, 2024
99. Antibody-Proteolysis Targeting Chimera Conjugate Enables Selective Degradation of Receptor-Interacting Serine/Threonine-Protein Kinase 2 in HER2+ Cell Lines
November 2, 2023
98. Glutathione Mediates Control of Dual Differential Bio-orthogonal Labelling of Biomolecules
October 31, 2023
97. Profiling Sulfur(VI) Fluorides as Reactive Functionalities for Chemical Biology Tools and Expansion of the Ligandable Proteome
January 17, 2023
96. Fluorous-Directed Assembly of DNA Origami Nanostructures
December 20, 2022
95. Surface Passivation with a Perfluoroalkane Brush Improves the Precision of Single-Molecule Measurements
October 28, 2022
94. Oligonucleotide-Recognizing Topoisomerase Inhibitors (OTIs): Precision Gene Editors for Neurodegenerative Diseases?
September 29, 2022
93. Biophysical characterisation of the Bcl-x pre-mRNA and binding specificity of the ellipticine derivative GQC-05: Implication for alternative splicing regulation
August 17, 2022
92. Biosynthesis of Aurodox, a Type III Secretion System Inhibitor from Streptomyces goldiniensis
July 18, 2022
91. A Chemo- and Regioselective Tandem [3 + 2]Heteroannulation Strategy for Carbazole Synthesis: Combining Two Mechanistically Distinct Bond-Forming Processes
March 18, 2022
90. Direct, Late-Stage Mono-N-arylation of Pentamidine: Method Development, Mechanistic Insight, and Expedient Access to Novel Antiparastitics against Diamidine-Resistant Parasites
August 6, 2021
89. A Phenotypic Approach for the Identification of New Molecules for Targeted Protein Degradation Applications
May 27, 2021
87. Contra-thermodynamic E → Z isomerization of cinnamamides via selective energy transfer catalysis
December 18, 2020
88. Glasgow Early Treatment Arm Favirpiravir (GETAFIX) for adults with early stage COVID-19: A structured summary of a study protocol for a randomised controlled trial
November 19, 2020
83. Mycobacterium tuberculosis Decaprenylphosphoryl-β-d-ribose Oxidase Inhibitors: Expeditious Reconstruction of Suboptimal Hits into a Series with Potent in Vivo Activity
October 1, 2020
85. Identification of 2-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)amino)-N-phenylpropanamides as a novel class of potent DprE1 inhibitors
June 15, 2020
86. Biocatalytic Alkylation Cascades: Recent Advances and Future Opportunities for Late-Stage Functionalization
May 27, 2020
84. Two-dimensional infrared spectroscopy: an emerging analytical tool?
February 10, 2020
77. Sequence-Selective Minor Groove Recognition of a DNA Duplex Containing Synthetic Genetic Components
May 22, 2019
74. Organic Semiconductor Laser Platform for the Detection of DNA by AgNP Plasmonic Enhancement
September 18, 2018
70. Effect of oligomer length on vibrational coupling and energy relaxation in double-stranded DNA
August 17, 2018
66. Modular, Step-Efficient Palladium-Catalyzed Cross-Coupling Strategy To Access C6-Heteroaryl 2-Aminopurine Ribonucleosides
July 6, 2017
62. A Tandem Enzymatic sp2-C-Methylation Process: Coupling in Situ S-Adenosyl-l-Methionine Formation with Methyl Transfer (ChemBioChem 11/2017)
May 15, 2017
65. Synthetic biological approaches for RNA labelling and imaging: design principles and future opportunities
May 8, 2017
64. Strategy for Conditional Orthogonal Sequential CuAAC Reactions Using a Protected Aromatic Ynamine
April 28, 2017
63. Ultrafast 2D-IR and optical Kerr effect spectroscopy reveal the impact of duplex melting on the structural dynamics of DNA
April 6, 2017
61. Reversible DNA micro-patterning using the fluorous effect
February 23, 2017
60. Determining the Origin of Rate-Independent Chemoselectivity in CuAAC Reactions: An Alkyne-Specific Shift in Rate-Determining Step
February 16, 2017
59. Transition-Metal-Free Amine Oxidation: A Chemoselective Strategy for the Late-Stage Formation of Lactams
February 8, 2017
49. SERS enhancement of silver nanoparticles prepared by a template-directed triazole ligand strategy
June 13, 2015
46. Defining the Structural Parameters of Triazole Ligands in the Templated Synthesis of Silver Nanoparticles
August 15, 2014
42. An oligofluorene truxene based distributed feedback laser for biosensing applications
April 15, 2014
44. Malaria Protein Kinase CK2 (PfCK2) Shows Novel Mechanisms of Regulation
March 24, 2014
35. Orthogonal, metal-free surface modification by strain-promoted azide–alkyne and nitrile oxide–alkene/alkyne cycloadditions
June 13, 2012
34. Directed Assembly of DNA-Functionalized Gold Nanoparticles Using Pyrrole–Imidazole Polyamides
May 7, 2012
33. Photo-induced growth of DNA-capped silver nanoparticles
March 2, 2012
32. Triazoles from N-Alkynylheterocycles and Their Coordination to Iridium
January 25, 2012
30. DNA-Templated Photonic Arrays and Assemblies: Design Principles and Future Opportunities
June 10, 2011
29. Site-Specific Assembly of DNA-Based Photonic Wires by Using Programmable Polyamides
February 25, 2011
27. Cu-Catalyzed N-Alkynylation of Imidazoles, Benzimidazoles, Indazoles, and Pyrazoles Using PEG as Solvent Medium
January 4, 2010
26. Highly Efficient Synthesis of DNA-Binding Hairpin Polyamides via the Use of a New Triphosgene Coupling Strategy
August 11, 2009
24. Chain-like assembly of gold nanoparticles on artificial DNA templates via ‘click chemistry’
November 29, 2007
4. New labelling strategies for the sensitive detection of nucleic acids
January 1, 2005
23. Pronounced Effect of DNA Hybridization on Click Reaction Efficiency
22. Synthesis of Highly Modified DNA by a Combination of PCR with Alkyne-Bearing Triphosphates and Click Chemistry
21. Transfer Printing of DNA by “Click” Chemistry
19. Formation of Bimetallic Ag/Au-Nanowires by Metallization of Artificial DNA-Duplexes
18. Click chemistry as a reliable method for the high-density functionalisation of alkyne-modified oligodeoxyribonucleotides
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